Composite

Part:BBa_K3089011:Design

Designed by: Zhao Xuanyu   Group: iGEM19_Greatbay_SCIE   (2019-10-14)


T7 promoter+csgA-linker-mfp5-His fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 76
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GGGGSGGGGS linker was added between different proteins and His-tag was fused on the C-terminal for affinity purification.


Source

CsgA is an amyloid-like protein encoded on the genome of E.coli MG1655 providing mechanical cohesive strength and Mfp5 is a mussel foot protein from Mytilus galloprovincialis responsible for interface adhesion.

References